Table 1 Summary of donor information. Clinical information (age, sex, cause of death), days between death to cell isolation, and cell cumulative population doubling (cpd) times of NCMs and RPE cells. f: female, m: male. n.d.: not determined. NCM cpd with †: cpd of NCM culture estimated based on the splitting ratio during passages and the last passage in culture. The cultures were used up for experiments before proliferation ceased (indicated by “ > ”) or deliberately terminated due to ceased proliferation before reaching confluence (indicated by “ < ”). RPE cell cpd with †: cells analyzed as previously published [37]

1

NCM602

RPE601

77/f

Pulmonary cancer

4

 > 10†

Not attached

2

NCM608

RPE607

64/f

Cerebral aneurysm rupture

4

 > 10†

11.19†

3

NCM709

RPE708

68/f

Pulmonary cancer

2

 > 8†

9.35†

4

NCM713

RPE712

77/m

Malignant arrhythmia

4

 > 11†

7.85†

5

NCM714

RPE713

84/f

Cardiogenic shock

3

 > 3†

n.d

6

NCM730

RPE729

65/f

Pulmonary embolism and pulmonary cancer

5

 > 5†

11.15†

7

NCM70Y

RPE70Y

70/m

Pulmonary adenocarcinoma

4

 > 8†

n.d

8

NCM71Y

RPE71Y

71/m

Esophagus cancer

3

Bacterial contamination

Bacterial contamination

9

NCM76X

RPE76X

76/f

Metastatic pulmonary neoplasia

3

 > 8†

9.38†

10

NCM85Y

RPE85Y

85/m

Pulmonary cancer

5

 < 2†

Bacterial contamination

11

NCM80Y

RPE80Y

80/m

Squamous cell carcinoma (tongue)

3

 < 2†

n.d

12

NCM1009

RPE1008

74/f

Peritoneal cancer with brain death

5

 > 10†

n.d

13

NCM720

RPE719

65/m

Not provided

6

9.96

n.d

14

NCM809

RPE808

69/m

Generalized cancer

4

10.98

15.41

15

NCM830

RPE829

73/m

Not provided

4

2.13

19.81

16

NCM922

RPE921

74/m

Not provided

5

2.26

Bacterial contamination

17

NCM1006

RPE1005

71/f

Not provided

5

9.58

Bacterial contamination

18

NCM114

RPE113

76/f

Pulmonary carcinoma

3

6.68

32.35

19

NCM323

RPE322

70/m

Glioblastoma

5

12.37

20.65

20

NCM66Y

RPE66Y

66/m

Pulmonary cancer

2

n.d

n.d