Fig. 5

Transcriptional activation ability of AsWRKY9. A Schematic view of the plasmid combinations of LUC reporters and effector. The promoter fragments of  AsFMO1 were cloned into the pGreenII 0800-LUC vector to generate the reporter constructs. The effector was generated by recombining the AsWRKY9 gene into the pHB-GFP vector. LUC, firefly luciferase; REN, Renilla luciferase. B Effects of AsWRKY9 on the promoter activity of AsFMO1as demonstrated by luciferase reporter assay. C Quantitative analysis of luminescence intensity. Three biological replicates were performed. The p-values were evaluated using Student’s t-test. Stars indicate the level of significance, *0.01 < p < 0.05, and **p < 0.01