Fig. 4

ChIP-qPCR and EMSA analysis. A Schematic representation of the AsFMO1 promoter region and its key regulatory element. The W-box motif is highlighted in red. The CDS of AsFMO1 is indicated in blue. B ChIP-qPCR analysis demonstrating the specific enrichment of the W-box region compared to the CDS region. Error bass represent SD for three independent experiments. C EMSA showing the interaction between AsWRKY9 and the AsFMO1 promoter. The free probe is indicated at the bottom of the gel. The competitor probe sequences were the same as the labeled probes but without biotin labeling