Fig. 2

Sequence analysis, tissue expression pattern analysis, and subcellular localization of AsWRKY9. A Protein sequence alignment of AsWRKY9 and its closest homologs from different plant species. The WRKY domain and zinc-finger motif are represented with red lines. B Phylogenetic tree of AsWRKY9 with its homologs from the selected plant species. C The expression patterns of AsWRKY9 in root, leaves, stalk, and bulb tissues of Allium sativum L. D Subcellular localization of AsWRKY9 N. benthamiana leaves epidermal cells were transformed with the fusion construct (AsWRKY9-GFP) and the nuclear marker NLS-mCherry. Bars = 20 μm