Fig. 4

Integrin β1 knockdown inhibits high ECM stiffness-increased plectin expression and HCC cell migration. A Western blot analysis was conducted to assess the efficiency of integrin β1 knockdown in cells, along with plectin expression, followed by quantitative analysis. B Following integrin β1 knockdown, Western blot analysis was performed to investigate the effect of ECM stiffness on plectin expression. C Wound healing assays were conducted to analyze migration of MHCC-97H and MHCC-97L cells after integrin β1 knockdown. Data are presented as mean ± SD; n = 3, * p < 0.05, ** p < 0.01, Scale bar: 200 μm