Table 3 Primers used in this study
From: Zinc finger proteins facilitate adaptation of a global insect pest to climate change
Primer | Primer sequence (5′−3′) |
|---|---|
PxZNF568-CDS-F | ATGGCTGACCCTTTAGTGCTGC |
PxZNF568-CDS-R | TTAATGCCTGTGTATTTTACCAAAACT |
PxZNF93-CDS-F | ATGGAGGAAAGCGTTCGGAA |
PxZNF93-CDS-R | TTACTTACTTTCTTTAAGTTTATGATGAATAAAT |
PxZNF266-CDS-F | ATGACGACGATAAACACAAAGATGT |
PxZNF266-CDS-R | CTAAAATCCTTCATTCTCATGTGAACG |
Q-PxZNF568-F | CGAAGAGCGATACCAACAGAA |
Q-PxZNF568-R | TTTCTTCAGTTCGCAGGAGAG |
Q-PxZNF93-F | AGGGAGTGTGAGAGAGAGAAA |
Q-PxZNF93-R | GGGAACTGCTGTACATGTCTTA |
Q-PxZNF266-F | TGAGACAGACACACGGTAATAAG |
Q-PxZNF266-R | GCTTCTTCAGGCTATGGTTAGT |
Q-PxRPL32-F | CAATCAGGCCAATTTACCGC |
Q-PxRPL32-R | CTGCGTTTACGCCAGTTACG |
PxZNF568-SgRNA-Fa | TAATACGACTCACTATAGGAATAGACGACTACGTAACCAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC |
PxZNF93-SgRNA-Fa | TAATACGACTCACTATAGGAAAACTTGATGTGCGACGCGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC |
PxZNF266-SgRNA-Fa | TAATACGACTCACTATAGGGAGATCCGTATCGCCAGTCAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC |
sgRNA-Com-Rb | AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAA |