Fig. 1

Single-cell clustering, APA events, and differential expression analysis. a UMAP plot showing single-cell clustering post batch effect correction, colored by sample origin. b UMAP plot depicting cell clustering results, colored by annotated cell types. c UMAP visualization of cell clusters and colored based on clustering results applied on APA matrix. d Histogram indicating the number of APA sites per gene. e Heatmap showing the overlap between APA and gene expression clusters. f Bar chart comparing the total differential expression event counts for each cell cluster vs. all other cell clusters. g Volcano plots showing differential gene expression differences between cell types. h Visualization of APA site variation for gene GBR2 across OPCs and neoplastic cells generated from BAM files