Fig. 6

The effect of developmental and adult temperature on trehalose and glucose content in adult (8-day-old) flies. Trehalose and glucose levels were significantly influenced by both developmental and adult temperatures, though the changes in these circulating carbohydrates were smaller compared to those observed for fat and glycogen. However, a notable reduction in trehalose and glucose levels occurred at high adult temperatures (33 °C). The amounts of trehalose and glucose are expressed as µg per fly. The amounts normalized to protein content (expressed as µg per mg protein) are depicted in Additional file 1: Fig. S9. Contour lines were computed using Delaunay triangulation, and the contour plot was smoothed with the Thin Plate Spline algorithm (OriginPro 2024 software). For statistical analyses, see Additional file 1: Tables S5, S6