Fig. 5

Systemic metabolite changes after repeated intermittent fasting and refeeding. A Experimental design: C57BL/6 J mice were either fed a standard diet and sacrificed on day 15 (FED, n = 4), intermittently fasted (IF, n = 3) and sacrificed on day 12, immediately after fasting, or on day 15 after re-feeding (RF, n = 4). B Body weight, food and water intake during the experiment. C Plasma was collected after the last day of the experiment, before the mice were sacrificed. sPLS-DA analysis of the NMR data. D Glucose, 3-HB, acetone and lactate concentration in the plasma of mice. E Branched-chain amino acid concentrations in the plasma of mice. Significant differences were analysed by two-way ANOVA with Bonferroni post hoc tests. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***); *IF/FMD vs FED, ^&IF vs FMD. Individual data values can be found in Additional file 9: Table 5