Fig. 3

Genome-wide transcriptional reprogramming in response to acute dietary restriction. A Venn diagram illustrating the number of differentially expressed genes that are common and distinct in FMD-fed and IF mice; B Heatmap of differentially expressed genes (DEGs) in the liver of IF mice vs the mice fed standard diet (749 genes upregulated and 845 genes downregulated) as well as C mice fed FMD vs mice fed standard diet (855 upregulated genes and 904 downregulated genes) with upregulated transcripts shown in red and downregulated transcripts shown in blue. Values are presented as transcripts per million (TPMs); false discovery rate (FDR) 5%. DEGs were identified by comparing expression levels of each gene between groups; D Gene Set Enrichment Analysis (GSEA) for “KEGG fatty acid degradation” and “KEGG PPAR signalling pathway” in IF mice compared to mice fed standard diet as well as E mice fed FMD compared to mice fed standard diet. Dr.Tom analysis tool (BGI) was used to map GSEA hallmark gene sets (NES, normalized enrichment score); F Volcano plot showing DEGs with a fold change ≥ 1 and FDR 5% between IF and FMD groups; G KEGG pathway analysis of genes differentially upregulated in IF compared to FMD-fed mice. X-axis shows the enrichment factor and the y-axis indicates the associated KEGG pathway. The size of the dots reflects the number of differentially expressed genes associated with each pathway, and the colour of the dots indicates the q values