Fig. 1

Intermittent fasting and fasting-mimicking diet in mice. A Experimental design: C57BL/6 J mice were either fed standard diet ad libitum (FED, n = 6), intermittently fasted (IF, n = 6) for 24 h on days 1 and 4, or fed a fasting-mimicking diet (FMD, n = 8) on days 2–4. Animals were sacrificed on day 5. B Macronutrient composition of the standard diet and FMD. C Body weight, food and water intake during the 5 days of the experiment. D Representative Oil Red O, PAS and H&E staining of the livers from fed, IF and FMD-fed mice