Fig. 3
From: Long-term survival of asexual Zymoseptoria tritici spores in the environment
Assessment of glycogen (A) and trehalose (B) concentrations over time in Z. tritici cells suspended in water. Blastospores were treated with either Aspergillus niger α-amyloglucosidase (A) or porcine trehalase (B). Glucose liberated from each reaction was assayed using a Glucose (GO) Assay Kit. Controls were not treated with either enzyme and so reflect the native free glucose content of Z. tritici cells. Sample optical density was measured at 540 nm using a spectrophotometer and compared against prepared glucose standards. Data are means of two experiments, each containing three replicate samples (2 samples on day 8 only). Circles represent overall means; small crosses show means from each experiment