Fig. 1

Assessment of Z. tritici blastospore populations over 49 days suspended in water. A Percentage viable blastospores (spores with at least one live cell; assessed by live/dead staining with 0.05% (w/v) propidium iodide). B Percentage culturability—number of colonies from plating 100 blastospores as quantified by haemocytometer. C Blastospores expressing cytoplasmic ZtGFP (green) were stained with propidium iodide (pink) after 5 days in water. White arrow highlights an example dead cell; yellow arrows highlight instances of budding growth. D Mean number of cells per blastospore. E Total blastospore count of populations over time using a haemocytometer. In all experiments, data at each time point are means of counts from 4 independent experiments, each containing at least 4 confocal images totalling at least 100 blastospores (A, D); or 3 spread plates (B) or 3 haemocytometer counts (E). Error bars show SE