Fig. 5
Hoxa5 alleviates calcium distortions and improves insulin resistance. A After 5 h of fasting, insulin (0.5 U/kg) was injected intraperitoneally; after 30 min to collect iWAT, immunoblotting analysis was performed on p-AKT/AKT (56 kDa). B iWAT was collected from each group of mice after overexpression/interference of Hoxa5; immunoblotting analysis was performed on p-AKT/AKT (56 kDa) and p-GSK-3β/GSK-3β (47 kDa). C iWAT was collected from each group of mice after overexpression/interference of Hoxa5; immunoblotting analysis was performed on IP3R1 (314 kDa) and VDAC (32 kDa). D After overexpression/interference of Hoxa5 on mouse primary adipocytes, the cells were treated with palmitic acid with the addition of KRX-0401; immunoblotting analysis was performed on p-AKT/AKT (56 kDa), p-GSK-3β/GSK-3β (47 kDa), IP3R1 (314 kDa), and VDAC (32 kDa). E After overexpression/interference of Hoxa5 on mouse primary adipocytes, the cells were treated with palmitic acid with the addition of KRX-0401, stained with Fluo-3, AM Calcium probe, and visualized using an IFM. F The iWAT was collected, mitochondrial fractions were extracted, and Ca2+ levels were detected using the tissue intramitochondrial calcium ion concentration fluorescence quantification assay kit, and relative values were calculated by taking the level of mitochondrial protein Cox-2 as an internal reference. G Liver was collected from each group of mice after overexpression/interference of Hoxa5; immunoblotting analysis was performed on p-AKT/AKT (56 kDa). n = 4 in each group. Values are mean ± SD. *p < 0.05, **p < 0.01. iWAT inguinal white adipose tissue, HFD high-fat diet, IFM inverted fluorescence microscope