Fig. 5

Bacterial metabolic pathways correlated with C. elegans developmental rate and longevity. a, Bacterial metabolic pathways correlated with C. elegans egg-laying time. The pathways were sorted by the importance of random forest regression analysis. Egg-laying time of C. elegans, Gram-stain, and phylum/class are indicated by several annotation columns at the top of the matrix. b, Bacterial metabolic pathway correlated with C. elegans lifespan. The presence of metabolic competence (dark purple in the right panel) was associated with the mean lifespan of L. marina (cyan color in the left panel; darker colors indicated increased mean lifespan). Different bacterial genera are indicated by the different colors of the strain names. c–e, Metabolite supplementation in strains HQB-118 (c), HQB-123 (d) and HQB-263 (e) demonstrates their effects on C. elegans egg-laying time, which is proxies of developmental rates. Statistical analyses were performed by one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test, ***P  < 0.001. n = 3 independent experiments, each scoring 35 animals. Source data of c–e are presented in Additional file 6. Boxes indicate the first and third quartile of counts, lines in boxes indicate median values, and whiskers indicate 1.5 × IQR in either direction