Grb7, Grb10 and Grb14, encoding the growth factor receptor-bound 7 family of signalling adaptor proteins have overlapping functions in the regulation of fetal growth and post-natal glucose metabolism

Table 1 Grb7, Grb10 and Grb14 distribution in the developing mouse embryo

Tissue	Grb7		Grb10		Grb14
Tissue	Details	E	Details	E	Details	E
CNS	No	-	Yes*, ventral spinal cord, mid-brain	M	No	-
Ch plexus	inconclusive	-	Yes, epithelium	H	Yes, apical epithelial surface	H
Pituitary	Yes	H	Yes	M	No	-
Submandib	Yes, epithelium	H	No	-	No	-
Inner ear	Yes, epithelium	H	Yes	M	Yes	M
Nasal ep	Yes	H	Yes	M	Yes	M
Tooth prim	Yes	M	Yes	M	Yes	M
Cartilage	Yes, ossified	V	Yes	H	Yes, ossifying	H
Sk muscle	No	-	Yes, widely	H	Yes, intercostal, diaphragm	H
Heart	Yes, Pericardium	L	Yes, Cardiac muscle	M	Yes, Cardiac muscle	H
Lung	Yes, epithelial	H	Yes, epithelial	H	Yes, epithelial	M
liver	Yes	H	Yes	H	Yes	M
Pancreas	Yes, endocrine	H	Yes, endocrine	H	Yes, endocrine	M
Kidney	Yes, epithelial	H	Yes, epithelial	H	No	-
Adrenal gl	Yes, cortex	L	Yes, cortex	M	No	-
Stomach	Yes, epithelial	H	Yes, epithelial	H	Yes, epithelial	L
Lower gut	Yes, epithelial	H	Yes, epithelial	H	Yes, epithelial	V
Epidermis	No	-	Yes	M	Yes	M

Expression determined by immunohistochemistry on wild type embryo sections at e14.5. Expression is compared in the listed tissues with notes on the specific localisation of each protein. An indication of relative expression level (E) is provided, in which levels have been judged separately for each antibody through comparison of tissues in the same sections (H = high, M = medium, L = low, V = very low). Grb10 is expressed in the CNS exclusively from the paternally inherited allele (indicated *) and in all other sites exclusively or primarily from the maternally inherited allele. Data was gathered from multiple stained embryos (a minimum of three for each antibody). Ch plexus = choroid plexus; gl = gland; Nasal ep = nasal epithelium; Sk muscle = skeletal muscle; Submandib = submandibular salivary gland; Tooth prim = tooth primordium

ISSN: 1741-7007