Fig. 4

Weights and blood glucose levels in PN1 progeny from Grb10 KO x Grb14 KO crosses. Weights of whole body and selected dissected organs, with blood glucose levels were collected at PN1 from progeny of crosses between Grb10 KO and Grb14 KO mice. Data were pooled into four groups for analysis as described in the Methods; wild type (WT), Grb10 KO (10KO), Grb14 KO (14KO) and Grb10:Grb14 double knockout (DKO). For each of the four offspring genotype groups, data are shown for (A) Body weight; and B Blood glucose concentration ([Glu]). In addition, actual weights of (C) Brain; D Liver; E Lungs; F Heart; and G Kidneys; are shown above the relative weights of the same organs, expressed as a percentage of body mass (H–L). Values represent means and SEM, tested by one-way ANOVA using Kruskal–Wallis and Dunn’s post hoc statistical tests. Sample sizes were, for total body and all organs, WT N = 25, Grb10 KO N = 13, Grb14 KO N = 7, Grb10:Grb14 DKO N = 7; glucose levels, WT N = 18, Grb10 KO N = 9, Grb14 KO N = 6, Grb10:Grb14 DKO N = 4. Asterisks indicate p-values, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001