Fig. 5

C. elegans NP-GPCRs with pan-phylum representation in nematodes. a Among the top 20% of the conserved C. elegans NP-GPCRs that have at least one ortholog in most species, seven (NPR-9, FRPR-5, NPR-16, C50H11.13, F13H6.5, F59D12.1, and NPR-19) are still orphan receptors. b Amino acid sequence alignment of AstA/buccalin-like peptides. Amino acids highlighted in green are conserved in all peptide sequences; amino acids in red match the C-terminal sequence motif [F/Y]XXG[L/I/V]G, characteristic of the AstA/buccalin peptide family [1]. Amino acids highlighted in gray are buccalin-specific. c Amino acid sequence alignment of AstC/somatostatin-like peptides. Amino acids in green are identical in all peptides; amino acids in orange are conserved in 60% of all peptide sequences. Aplysia californica (Aca), Acyrthosiphon pisum (Api), Caenorhabditis elegans (Cel), Crassostrea gigas (Cgi), Capitella teleta (Cte), Drosophila melanogaster (Dme), Daphnia pulex (Dpu), Helobdella robusta (Hro), Lottia gigantea (Lgi), and Tribolium castaneum (Tca)