Fig. 1

In silico pipeline for identifying C. elegans NP-GPCR orthologs in the nematode phylum. A resource of 161 known and predicted C. elegans NP-GPCRs was used to search for homologs in predicted proteomes of 125 nematode species using a Hidden Markov Model (hhmbuild, hmmsearch and hmmtop), yielding 15,929 protein sequences. The predicted NP-GPCRs were used in clustering and phylogenetic analyses to identify orthologs of C. elegans NP-GPCR candidates. Orphan NP-GPCR candidates of C. elegans that show broad conservation in the nematode phylum (C50H11.13, F13H6.5, F59D12.1, FRPR-5, NPR-9, NPR-16, and NPR-19) are prioritized for functional studies. MSA, Multiple Sequence Alignment; TM, TransMembrane; CLANS, CLuster ANalysis of Sequences