Fig. 9

Uptake of dsRNA by C. rosea and miRNA mimics by wheat roots. A GFP-tagged C. rosea conidia (C. rosea-GFP) was incubated with Cyanine 3-UTP labeled dsRNA (Cy3-dsRNACt) and examined under a confocal microscope at 24 h post incubation (hpi). Representative confocal microscopy images showing uptake of Cy3-dsRNA by C. rosea hyphae and conidia (left panel, magenta), C. rosea-GFP control (middle panel), and colocalization of GFP (Green) with Cy3 (Right panel) in Cy3-dsRNACt-treated C. rosea-GFP (right panel). B Representative confocal microscopy images show the uptake of Cy3-labeled miRNA mimics by wheat roots. The left panel shows a control treatment with no Cy3 fluorescence signal (Magenta). The right panel shows Cy3 miRNA 12061 mimic (Magenta) internalization into wheat roots (confocal microscopy images of root cross sections). For the experiment, artificially synthesized mir_17532_x1 miRNA mimic miR17532 was applied on wheat roots, and a Confocal microscopic analysis of the root surface and horizontal cross-section was performed 24 hpi