Fig. 1

Validation of FgCWM1 interaction with TaNDUFA9 and pTaNDUFA9. a Verify the interaction between FgCWM1 and TaNDUFA9 as well as pTaNDUFA9 using the Yeast two-hybrid system. Positive control: pGBKT7-53 + pGADT7-T; negative control: pGBKT7-Lam + pGADT7-T. DDO: SD/-Trp/-Leu. QDO/X/A: SD/-Trp/-Leu/-Ade/-His/X-α-gal/3-AT. b Relative expression of TaNDUFA9 in wheat spikes 48 h after F. graminearum inoculation. “*” above each box indicates significance at P < 0.05 (n = 3). c Modeling of the tertiary structure of TaNDUFA9 protein. Pink represents the pTaNDUFA9 region, while green represents the transmembrane domain. d Verification of the interaction of FgCWM1 with TaNDUFA9 and pTaNDUFA9 by Co-IP. “ + ” represents the addition of the corresponding protein; “-” represents the absence of the corresponding protein. “Anti-MYC” was incubated with the MYC antibody, and “Anti-GFP” was incubated with the GFP antibody. Input refers to total protein, which belongs to the positive control. “IP” refers to the precipitation of the corresponding protein with the corresponding antibody, and “IB” refers to the interaction signal. The original picture is displayed in Additional file 1: Fig. S3. e Verification of the interaction of FgCWM1 with TaNDUFA9 and pTaNDUFA9 by BiFC. “BF” represents a bright field of vision, “AF” represents chloroplast autofluorescence, “GFP” represents the green fluorescence signal, and “Merge” represents a composite map with BF, AF, GFP, and RFP. Scale bar = 20 μm