Fig. 3

SOX7/17 expression depends on MEK1/2 signalling in the developing testis. Section or wholemount immunofluorescent imaging of E12.5 testes cultured for 24 or 72h with DMSO or 500nM of MEKi. A and C Section IF of cultured testes stained with DAPI (blue), SOX7/17 (red) and CD31 (endothelial cells and germ cells; cyan). B Three-dimensional stacked Z-series images of cultured testes showing Oct4-eGFP (germ cells; green), SOX7/17 (blue) and pERK1/2 (red). The DMSO and MEKi images should be viewed in conjunction with Movies S5-8 which reveal the 3D organisation of the cells in the tissue. Scale bar represents 100 μm. Arrows indicate SOX7/17 positive endothelial cells. Biological replicates; n = 3–4 per treatment