Fig. 2

MEK1/2 inhibition depletes pERK1/2 and CD31 expressing endothelial cells in developing testes. Section or wholemount immunofluorescent imaging of E12.5 testes cultured for 24 or 72h with DMSO or 500nM of MEKi. A and C Section IF of cultured testes stained with DAPI (blue), pERK1/2 (red) and CD31 (endothelial cells and germ cells; cyan). B Three-dimensional stacked Z-series images of cultured testes showing Oct4-eGFP (germ cells; green), CD31 (endothelial cells and germ cells; blue) and pERK1/2 (red). The DMSO and MEKi images should be viewed in conjunction with Additional files 4–7: Movies S1-4, which reveal the 3D organisation of the cells in the tissue. Scale bar represents 100 μm. Arrows indicate pERK1/2 positive endothelial cells and asterisks indicate pERK1/2 positive Sertoli cells. Biological replicates; n = 3–4 per treatment