Fig. 5

Functional verification of driver gene TNC in odontoblastic differentiation. a mRNA expression of TNC after 7 days and 14 days of odontogenic induction. b Protein expression of TNC after 7 days and 14 days of odontogenic induction. c mRNA expression of TNC in hDPSCs transduced with shCtrl, shTNC1, or shTNC2 lentivirus. d Representative western blots showing the protein level of TNC in hDPSCs transduced with shCtrl, shTNC1, or shTNC2 lentivirus. e mRNA expression of TNC, RUNX2, and DSPP in hDPSCs transduced with shCtrl and shTNC2 lentivirus after 7 days 14 days of induction of differentiation. f Representative western blots showing the protein level of TNC, RUNX2, and DSPP in transduced hDPSCs after 7 days and 14 days of induction of differentiation. g Representative bright-field microscopy images of ARS of transduced hDPSCs at 14 days after the induction of differentiation. The data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001