Fig. 4

Immunohistochemistry for the detection of Tnc expression in the rat mandibular first and second molar. a, c, e Representative images of the expression of Tnc in the rat mandibular first and second molars at PN1, PN5, and PN10, respectively. Tnc is mainly expressed in odontoblasts and dental pulp cells. b, d, f Images of the region of interest from the red box in a, c, e. Positive signals are indicated by red arrows. Scale bars for a, c, e indicate 500 μm, and for b, d, f indicate 100 μm. e enamel, d dentin, od odontoblast cells, p pulp cavity