Fig. 8
Maternal uterine physiology is altered in response to paternal diet. A The number of CD31+ blood vessels per mm2 of uterine tissue and (B) the average area of blood vessels across whole uterine Sect. (100 vessels measured per section). C Representative images of CD31+ blood vessels in the uterus on day 3.5 of gestation from females mated with CD, LPD, MD-LPD, WD and MD-WD males. D The proportional distribution of blood vessel sizes from imaged tissue, categorised into small (< 100 μm2), medium (100–250 μm2) or large (> 250 μm2) area vessels. E The number of uterine glands per mm.2 of uterine tissue (CD n = 7, LPD n = 5, MD-LPD n = 6, WD n = 6, MD-WD n = 6). F Whole uterus gene expression of key immune regulatory and inflammatory genes presented relative to CD expression, normalised using GeNorm method against Tbp and Ppiβ. Data points represent average for one dam (CD n = 7, LPD n = 7, MD-LPD n = 7, WD n = 7, MD-WD n = 7) with average ± SEM. Statistical significance determined using one-way ANOVA with Tukey’s post-test; *p < 0.05