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Fig. 7 | BMC Biology

Fig. 7

From: Hypoxia and Foxn1 alter the proteomic signature of dermal fibroblasts to redirect scarless wound healing to scar-forming skin wound healing in Foxn1−/− mice

Fig. 7

Transduction efficiency of LVs carrying eGFP (control), Hif-1α-eGFP or Foxn1-mCherry + Hif-1α-eGFP injected into the wounded skin of Foxn1−/− mice at postwounding day 1 analysed by flow cytometry at day 14 or by immunohistochemistry for mCherry (LV-Foxn1-mCherry) at postwounding day 6. A Scheme of experiment. B Percentage of cells positive for eGFP or mCherry isolated from the injured skin of LV-eGFP (control)-, LV-Hif-1α-eGFP- or LV-Hif-1α-eGFP + LV-Foxn1-mCherry-injected Foxn1−/− mice. C–E Percentage of E-cadherin (E-cad +), vimentin (Vim +) or E-cadherin + vimentin double-positive cells (E-cad/Vim +) within eGFP-positive (LV-eGFP or LV-Hif-1α) or mCherry + eGFP-positive (LV-Hif-1α + LV-Foxn1) populations in comparison to the whole cell population. F Immunohistochemical detection of mCherry (LV-Foxn1-mCherry) localization in the skin of Foxn1.−/− mice. Asterisks indicate significant differences (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). Data represent the mean ± SD. E-epidermis, D-dermis, arrowheads indicate positivity in epidermis, arrows point out positivity in dermis (F). Scale bar (F) 50 μm, (inset; 20 μm)

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BMC Biology

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