Fig. 6

Impact of CgChs5 and CgChs6 on vegetative growth, asexual development, and virulence. A Colonies of the wild-type (WT), ΔCgchs5, ΔCgchs6, ΔCgchs5/CgCHS5, and ΔCgchs6/CgCHS6 strains on CM and PCA plates at 28ºC for 4 days in the dark. B Statistical analysis of the colony diameter for the indicated strains in (A). C Quantification of conidia produced by the indicated strains. D Lesions developed on wounded leaves of C. lanceolata inoculated with conidial suspensions of the WT, ΔCgchs5, ΔCgchs6, ΔCgchs5/CgCHS5, and ΔCgchs6/CgCHS6 strains for 5 days. E Protoplast release from the WT, ΔCgchs5, ΔCgchs6 strains after treatment with cell wall-degrading enzymes for 30 and 60 min at 30ºC. F Measurement of mycelial chitin content of the WT, ΔCgchs5, and ΔCgchs6 strains. Error bars represent standard deviation. Asterisks indicate a significant difference at P < 0.01