Fig. 5

Binding of CgCrzA to the promoter regions of CgCHS5 and CgCHS6. A, B Quantitative RT-PCR analysis of CgCHS5/6 expression in the ΔCgcrzA mutant and wild-type in response to CFW. HY and CO indicates hypha and conidia, respectively. Error bars represent standard deviation. Asterisks indicate a significant difference at P < 0.01. C and D Electrophoretic Mobility Shift Assay (EMSA) demonstrating the binding of CgCrzA^S280D protein to the putative promoter sequences of CgCHS5 (C) and CgCHS6 (D). The band shift of the promoter with increasing concentration of CgCrzA^S280D protein. GST indicates glutathione S-transferase tag protein. E and F Incubation of the Alex660-labelled full-length promoter DNA in the absence (leftmost lane) or presence (second to the sixth lane) of purified CgCrzA^S280D and GST proteins (rightmost lane). Unlabelled DNA was added as a binding competitor, with increasing amounts in the third to sixth lanes