Fig. 3
From: Phospho-code of a conserved transcriptional factor underpins fungal virulence
Vital roles of CgCrzA phosphorylation at Ser280 in virulence and cell wall integrity. A, B Disease lesions on wounded leaves of C. lanceolate, caused by the wild-type, ΔCgcrzA, ΔCgcrzA/CgCRZAS280A, ΔcgcrzA/CgCRZAS280D, ΔCgmkk1, ΔCgmkk1/CgCRZAS280A, and ΔCgmkk1/CgCRZAS280D strains at 5 days post-inoculation with conidial suspensions. C, D Chitin staining with CFW in the hyphae of the wild-type, ΔCgcrzA, ΔCgcrzA/CgCRZAS280A, ΔcgcrzA/CgCRZAS280D, ΔCgmkk1, ΔCgmkk1/CgCRZAS280A, and ΔCgmkk1/CgCRZAS280D strains. Bar = 10 μm. E, F Statistical analysis of protoplast release from the wild-type, ΔCgcrzA, ΔCgcrzA/CgCRZAS280A, ΔCgcrzA/CgCRZAS280D, ΔCgmkk1, ΔCgmkk1/CgCRZAS280A, and ΔCgmkk1/CgCRZAS280D strains after treatment with cell wall-degrading enzymes. G, H Growth inhibition ratio of the wild-type, ΔCgcrzA, ΔCgcrzA/CgCRZAS280A, ΔCgcrzA/CgCRZAS280D, ΔCgmkk1, ΔCgmkk1/CgCRZAS280A, and ΔCgmkk1/CgCRZAS280D subjected to CFW and CR, respectively. Error bars represent standard deviation. Asterisks indicate a significant difference at P < 0.01