Fig. 5

KIAA1429 repressed collagen synthesis in UVR-irradiated HDFs via m⁶A-MFAP4-mediated manner A MeRIP-qPCR was applied to assess the m⁶A modification of MFAP4 in control and UVR-irradiated HDFs transfected with or without si-KIAA1429. m⁶A-IP/input and IgG-IP/input were used to calculate the enrichment of m⁶A in each group (n = 7). B RT-qPCR detected the mRNA expression of MFAP4 in each group (n = 7). C The protein level of MFAP4 was identified using western blot in each group (n = 3). D Western blot of the expression of TGF-bRII and MMP1 in each group (n = 3). E Immunofluorescence assay of the colocalization of COL1a2 with MFAP4 in each group. Nuclear staining (DAPI) and merged images are also shown in the diagram. Scale bars = 75 μm. All data were presented as mean ± SD of three independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001