Fig. 4

KIAA1429 regulated collagen synthesis in UVR-irradiated HDFs. A The m⁶A levels of total mRNA in control and UVR-irradiated HDFs were detected using m⁶A dot blot. B mRNA expression of KIAA1429 was identified by RT-qPCR between the two groups (n = 7). C The protein level of KIAA1429 in two groups was detected using western blot (n = 3). D RT-qPCR detects the effect on knockdown of KIAA1429 expression by siRNA in the HDFs cells (n = 7). E The m⁶A levels content of mRNA were identified by m⁶A dot blot in UVR-irradiated HDFs with or without KIAA1429 knockdown. F The protein levels of TGF-bRII and MMP1in control and UVR-irradiated HDFs transfected with or without si-KIAA1429 were measured using western blot (n = 3). G Immunofluorescence staining was performed with anti-COL1A2 in each group. Nuclear staining (DAPI) and merged images are also shown in the diagram. Scale bars = 75 μm. All data were presented as mean ± SD of three independent experiments. ^**p < 0.01, ^***p < 0.001