Fig. 1
From: The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis
Global m6A levels and methyltransferases KIAA1429 expression were significantly upregulated in UVR-induced photoaged skin. A The clinical manifestations of dorsal skin in the control and UVR group. B HE staining of skin in each group. Magnification: 100 × . C Masson stain of skin in each group. Magnification: 100 × . D SOD levels in each group by ELISA (n = 7). E MDA and Hyp levels in each by ELISA (n = 7). F Protein expression of TGF-bRII and MMP1 were evaluated by western blot (n = 3). G The m6A levels of total mRNA were determined by m6A dot blot in both groups. H The mRNA expression of METTL3, METTL14, METTL16, WTAP, KIAA1429, RBM15, RBM15B, ZC3H13, FTO and ALKBH5 were indicated by RT-qPCR (n = 7). I Protein level of KIAA1429 was detected by western blot in each group (n = 3). J IHC staining for KIAA1429 protein from skin tissue in each group (n = 7). Scale bar is 100 μm and 50 μm (magnified). All data were presented as mean ± standard deviation (SD) of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001