Fig. 6
HRJD expression in adult ISCs/EBs improves stem cell functions in aged midguts. A Representative images for esg-Gal4 positive cells in day 30 midguts. HRJDs suppressed mis-differentiation of esg+ cells (cellular enlargement with polyploid large nuclei). B Quantification of nuclear size of esg-Gal4 positive cells. C, D Representative images (C) and quantification (D) of PH3 positive cells in day 30 posterior midguts. E RT-qPCR of Dl, nub, and pros in day 30 midguts. Expression levels were normalized to those of GFP control. F, G Cell type composition in aged midguts were assessed with esgts > GFP (ISC, EB, and early EC), Dl-lacZ (ISC and EEP), and anti-Pros staining (EEP and EE). HRJD expression in adult ISCs/EBs reduced the ratio of EB/early EC and increased the ratio of EC (G). H, I esgts-mediated HRJD induction enhanced STAT-GFP intensity in Pros− diploid cells (ISCs/EBs), which is quantified in I. Additionally, HRJDs suppressed STAT activation in polyploid ECs (arrowheads). N.S., not significant: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, one-way ANOVAs with post hoc Tukey test. n indicates the number of cells (B, I), guts (D, G), and biological replicates (E). Scale bars: 50 μm