Fig. 5
HRJD expression in adult ISCs/EBs suppresses age-related gut barrier dysfunction. A esgts-mediated HRJD induction decreased Smurf( +) ratio at day 30. The representative images of the Smurf phenotype (leakage of blue dye throughout whole body) are shown on the left. B, C HRJD expression in adult ISCs/EBs maintained junctional localization of Dlg protein in ECs, which is quantified in C. D–I HRJD expression in adult ISCs/EBs maintained junctional localization of Tsp2A (D), Mesh (E), and Ssk (F) in ECs, which is quantified in G–I. J RT-qPCR of puc in the whole midgut. esgts-mediated induction of HRJDa significantly repressed puc. K, L puc-lacZ intensity in polyploid cells was decreased by HRJD expression (K), which is quantified in (L). *P < 0.05, **P < 0.01, ***P ≤ 0.001, one-way ANOVAs with post hoc Tukey test. n indicates the number of flies (A), cells (C, G, H, I, L), and biological replicates (J). Scale bars: 50 μm