Fig. 3

ARID1A deficiency slightly changes the editing level of the Q/R site of GlutR-B. A Schematic overview of the TA cloning process. After PCR amplification of a segment of the gene containing the Q/R site of GlutR-B, the fragment was inserted into the T-vector. After ligation and transformation, 100 single clones were selected and plated for Sanger sequencing. Sequence alignment was performed using SnapGene software. B Comparison of GlutR-B Q/R site sequences in edited state and non-edited state. The red arrow indicates the Q/R site. C The Q/R site editing rate in HCT116 ARID1A_WT and ARID1A_KO cell lines. n = 3 of independent experiments; ***, P < 0.001. D Western blot shows ARID1A knockdown efficiency in A2058 shARID1A cells. E The Q/R site editing rate in HCT116 ARID1A_WT and ARID1A_KO cell lines. n = 3 of independent experiments; ns, not significant. F Western blot shows ARID1A knockdown efficiency in A375 shARID1A cells. G The Q/R site editing rate in HCT116 ARID1A_WT and ARID1A_KO cell lines. n = 3 of independent experiments; ns, not significant