Fig. 4

Comparing global averaged DNA methylation by EM-seq methodology in three different motifs for MTase mutants demonstrates significant differences for the single, methylated motifs between wild type and isogenic MTase mutants. A Bar graph comparison of genome-wide conversion frequencies for the GCGC motif in N6 wild type strain (N6-wt [replicate R2]) and both isogenic MTase mutant strains (N6-gcgc [R2]; N6-tcttc [R1]). B Statistical differences for the aggregated genome-wide single-base quantitative results of GCGC motif methylation between strains (two biological and technical replicates [R1, R2] for each strain were performed and are summarized). C Bar graph comparison of genome-wide conversion frequencies for the CCTC motif in N6 wild type strain and both MTase mutants as in A. D Statistical differences for the aggregated genome-wide single-base results of CCTC motif methylation between strains (two biological and technical replicates each). E Bar graph comparison for the genome-wide conversion frequencies for the TCTTC motif in N6 wild type strain and both MTase mutants, replicates as in A. F Statistical differences for the aggregated genome-wide single-base quantitative results of TCTTC motif methylation between strains (two biological and technical replicates each, see also Table 1 for replicate numbers; Additional File 3: Table S2 for full single-base conversion data). Chi-square p-value * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. n.s. is non significant