Fig. 1

tPA is expressed by parvalbumin interneurons enwrapped with PNNs. A AAV-Plat-GFP was injected in the somatosensory cortex of adult wild-type mice. Co-immunohistochemistry of the GFP reporter (green) with the neuronal marker NeuN (magenta), the astrocyte marker GFAP (magenta), and the microglial marker Iba-1 (magenta) in the somatosensory cortex of adult WT mice 3 weeks after AAV injection. AAV-Plat-GFP induces expression of GFP in neurons but not in astrocytes or microglia (Scale bar: 100 μm). B,C Co-localization of the Plat-GFP reporter (green) with GABA (cyan) and parvalbumin (magenta) markers following injection of AAV-Plat-GFP in the somatosensory cortex of WT mice (Scale bar: 100 μm). Quantitative analysis of Plat-mediated expression of GFP in relation to GABAergic positive neurons and parvalbumin interneurons. C Around 37 % of Plat-GFP positive neurons are GABAergic interneurons and 73 % of them co-localized with PV marker (n=4 coronal sections from N=3 WT brains). D 3D reconstruction of a Plat-GFP positive neuron (green), co-labeled with PV (magenta) and the PNN marker Wisteria floribunda agglutinin (WFA, yellow) (Scale bar: 10 μm). E Quantification of Plat-GFP neurons double stained with PV and WFA