Fig. 5

Reelin effects on mushroom body neurons depend on LpR1 and LpR2. a–f Reelin does not affect neurite complexity in mutants for LpRs. a, d Sholl profile of cultured MB neurons from LpR1 (c309,eGFP/ + ;LpR1^DF/ +) or LpR2 (c309,eGFP/ + ;LpR2^DF/ +) mutant flies and their controls (c309,eGFP), after Mock or Reelin treatment (30 nM). Two-way ANOVA shows that treatment and genotype factors and their interaction play a role in results obtained for each data set (p < 0.0001 for all). “#” shows significant differences (p < 0.05) between the genetic control and mutants after Reelin treatment. “*” indicates significant differences between mutant neurons and genetic control after Mock treatment. “ + ” means a significant difference (p < 0.05) between mock and Reelin treatment in the mutant genotype. b, e Maximum length. c, f Critical value in the genotypes identified, after mock or Reelin treatment. In b, c, e, and f, two-way ANOVA shows that treatment and genotype factors, and also the interaction between factors, play a role in results (p < 0.0001 for each analysis); ** and ****, indicates p < 0.01 and p < 0.0001 between conditions. Data in a–f, from n = 24 coverslips, 15 cells studied in each coverslip, three independent experiments. g–l Reelin does not affect neurite complexity in LpRs knockdown flies. g, j Sholl profile, h, k maximum length, and i, l critical value measured in flies expressing RNAi for either LpR1 or LpR2 in MB neurons (c309,eGFP/ + ;RNAi-LpR1/ + or c309,eGFP/ + ;RNAi-LpR2/ + , respectively), as compared to controls (c309,eGFP), after Mock or Reelin treatment. In g, j, two-way ANOVA shows that treatment and genotype factors and their interaction play a role in results obtained for each data set (p < 0.0001 each). “#”, significant differences (p < 0.05) between control and LpRs knockdowns after Reelin treatment. “*”, significant differences between knockdown and control after Mock treatment. No significant differences were observed between mock and Reelin treatment in the knockdowns for LpRs. In h, two-way ANOVA indicates that only genotype and treatment factors play a role in results (p < 0.0001 and p = 0.0170 respectively). In k, two-way ANOVA indicates that genotype and treatment factors and their interaction play a role in results (p < 0.0001, p = 0.0034, and p = 0.0096 respectively). In i, two-way ANOVA indicates that only genotype and treatment factors play a role in results (p < 0.0001 and p = 0.0002 respectively). In l, two-way ANOVA indicates that genotype and treatment factors and their interaction play a role in results (p < 0.0001, p = 0.0073, and p = 0.0012 respectively). *, **, and **** indicated p < 0.05, p < 0.01, and p < 0.0001 respectively. Data in g–l, from n = 12 coverslips, 10–15 cells studied in each coverslip, three independent experiments. Data in j–l, n = 16 coverslips, 10–15 cells studied in each coverslip, four independent experiments. ns, not significant