Fig. 6

ccmFc encountered two independent breaks and became two genes. a Breakpoints in ccmFc. A long insertion in the second breakpoint meant that FR2 and FR3 are still adjacent in leafy species. In Cu. epilinum and Cu. europaea, the insertion is much shorter; the connection has broken in other Cuscuta. b, c Transcriptome coverage of the three fragments in sweet potato (I. batatas). Blue and red lines under the coverage indicated coverage less than 50 and greater than 400, respectively. The coverage was scaled by Log function in GENEIOUS. d Transcriptome coverage of the exon parts after the intergenic regions were removed. e Positions of primers and agarose gel analysis of RT-PCR confirming the gene fission. Gene length scales indicated on the tomato ccmFc. Lanes 1 to 7: Cu. australis, Cu. japonica, sweet potato, Dichondra, tomato, Arabidopsis, and no template