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Fig. 3 | BMC Biology

Fig. 3

From: Erv1 of Arabidopsis thaliana can directly oxidize mitochondrial intermembrane space proteins in the absence of redox-active Mia40

Fig. 3

Mitochondria expressing AtErv1 fail to import the Mia40 substrates Tim9, Cmc1, and CCMH. a–f The indicated proteins were synthesized in the presence of [35S]-methionine in reticulocyte lysate and incubated with mitochondria isolated from the indicated strains for the times indicated. Non-imported material was removed by treatment with proteinase K in all samples shown in this figure. Mitochondria were washed, re-isolated and subjected to SDS-PAGE and autoradiography; 10% of the radiolabeled protein used per time point was loaded as a control

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BMC Biology

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