Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species

Table 1 Genome assembly and annotation statistics

Species	H. armigera	H. zea	B. mori ^a	M. sexta ^b
Genome assembly	csiro4bp	csirohz5p5
Assembly size (Mb)	337.07	341.15	431.7	419.42
Number of scaffolds	997	2975	43,622	20,870
Max. scaffold length (Mb)	6.15	1.85	16.12	3.25
N50 scaffold size (kb)	1000.4	201.5	3717.00	664.01
N90 scaffold size (kb)	175.3	52.3	43.1	46.4
Mean scaffold length (kb)	338.1	114.7	9.9	20.1
Median scaffold length (kb)	117.3	68.0	0.655	0.997
Number of contigs	24,228	34,676	88,842	38,380
N50 contig length (kb)	18.3	12.6	15.5	40.4
Mean contig length (kb)	12.4	8.6	4.86	10.4
Median contig length (kb)	7.4	5.4	NA	NA
Gene annotation			(NCBI)^c
Protein-coding	17,086	15,200^d	15,007	27,404
InterPro domain	12,212	11,061	14,113	NA
GO	11,324	10,221	9462	NA
Pfam	10,700	9,795	11,753	NA
KEGG	4217	4004	6242	8611
Genomic features
Repeat (%)	14.6	16.0	43.6	24.9
GC (%)	36.1	36.2	38.8	35.3
Coding (%)	6.7	5.9	4.1	10.4
Intron (%)	39.3	17.7	16.3	NA
Gene length (b)	9098	5306	6029	NA
Avg. protein length (aa)	442.8	444.7	458.5	531.1
microRNAs	251	232	487	98
Quality control: BUSCO % present (complete)
Genome	94.3 (83)	93.2 (80)	91.6 (73)	93.7 (81)
Proteins (OGS)	94.6 (86)	90.7 (82)	93.6 (87)	92.9 (84)

N50 and N90 are computed on each assembly size as given in the table. The statistics for published B. mori and M. sexta genome assemblies are included for comparison, with references as follows:
^a B. mori v2 [39], ^b M. sexta [40], ^cNational Center for Biotechnology Information (NCBI) Gnomon models, ^dIndicates plus 1192 partial gene models
GO Gene Ontology, KEGG Kyoto Encyclopedia of Genes and Genomes, BUSCO Benchmarking Universal Single-Copy Orthologues, OGS official gene set

ISSN: 1741-7007